MICROSNAP™ COLIFORM

MicroSnap™ Coliform

MicroSnap™ Coliform is a rapid bioluminogenic test method designed for the detection and enumeration of coliform bacteria, delivering actionable same-day results. The MicroSnap™ test platform consists of an Enrichment Device containing a specific growth medium and a Detection Device containing a bioluminogenic (light-producing) substrate. When MicroSnap™ Coliform detects the target microorganisms, light is emitted and measured using a Hygiena™ luminometer. Results are securely stored and tracked with SureTrend™ Cloud software for data analysis, reporting, and trend monitoring.

MicroSnap™ Coliform is based on well-established bioluminescence technology widely documented in food safety and environmental microbiology literature as a reliable approach for rapid microbial enumeration. Industry validation studies and AOAC Performance Tested Methods have demonstrated strong correlation between relative light units (RLU) and traditional colony-forming unit (CFU) counts, supporting its use as an alternative to conventional plating methods. Customer and industry feedback frequently highlight the system’s speed, ease of use, and ability to support same-shift decision-making. The test is commonly used in food and beverage manufacturing, dairy processing, and environmental monitoring programs where rapid product release, trend analysis, and cost control are critical.

Key Benefits

• Same-shift actionable results
• Reduced testing and operational costs
• Simple, easy-to-use format
• Compatible with filtered samples
• Integrates with EnSURE™ Touch and SureTrend™ Cloud

Same-Shift Results

Rapid test results are essential for timely product release and corrective action. The MicroSnap™ family of rapid indicator organism tests enables food safety and quality managers to enumerate samples in as little as 6 hours and determine presence or absence within 8 hours. The workflow is straightforward: collect the sample, incubate, and read the result using a Hygiena™ luminometer.

Reduce Costs and Shorten Release Cycles

While external laboratory testing such as PCR analysis may be required for certain applications, many routine checks can be performed faster and more cost-effectively on-site. Incorporating MicroSnap™ Coliform as a rapid indicator test helps reduce material costs, shipping expenses, and laboratory fees. Faster results also shorten production hold times and lower inventory and warehousing costs.

Flexible and Easy-to-Use Format

MicroSnap™ Coliform is designed for flexibility in both environmental surface testing and product sample analysis. The system uses a simple two-step process: enrichment followed by detection. Its patented Snap-Valve technology delivers a precise volume of enrichment broth, while the built-in pipette allows easy transfer of enriched samples to the detection device. Results are read on a Hygiena™ luminometer, eliminating the need for manual colony counting. Outcomes are determined using an RLU-to-CFU conversion table.

Complete Hygiena Monitoring Program

MicroSnap™ Coliform plays a key role in a comprehensive environmental monitoring program. When combined with EnSURE™ Touch and SureTrend™ Cloud, test results can be captured, stored, and trended over time. The SureTrend™ Cloud dashboard allows users to correlate ATP hygiene data with microorganism results, providing deeper insight into sanitation effectiveness and risk reduction.

Works with Filtered Samples

MicroSnap™ Coliform & E. coli Enrichment Broth is available in a 2 mL vial format suitable for large-volume filtered samples. This configuration delivers same-day results and is particularly beneficial for beverage and liquid product processors seeking faster release times. The method is AOAC approved and validated against EPA reference methods.

MicroSnap™ Enhanced Nutrient Broth

MicroSnap™ Enhanced Nutrient Broth is a non-selective protein-based solution designed to support low-level enumeration. The 9 mL nutrient broth can be used with MicroSnap™ Total, MicroSnap™ Coliform, and MicroSnap™ E. coli tests. For milk samples, it enables detection down to 1 CFU/mL when used with MicroSnap™ Coliform, as validated by AOAC-RI Performance Tested Methods (Certificate #071302).

• Applications: Environmental and product testing
• Built-in pipette for easy sample transfer
• Snap-Valve technology – simply snap and squeeze
• Write-on swab label for sample identification
• MicroSnap™ Coliform detects:
• • E. coli
  • Klebsiella
  • Citrobacter
  • Enterobacter

To extend your rapid microbiological testing capabilities beyond the MicroSnap Coliform assay — which delivers same‑day enumeration of coliform bacteria in environmental and product samples — consider additional complementary tests that support a broader contamination control strategy. The MicroSnap E. coli test offers targeted detection and quantification of Escherichia coli to inform risk assessment and hygiene verification. For comprehensive assessment of viable organisms where total microbial load data is required, the MicroSnap Total – Total Viable Count test delivers same‑day TVC results to support sanitation effectiveness and product release decisions. Additionally, pairing these assays with a compatible luminometer such as the ATP Surface Sanitation Monitoring System with ENSURE TOUCH provides integrated data capture and reporting to enhance environmental monitoring and quality management workflows.

Q&A

What types of samples can be tested with MicroSnap™ Coliform?

MicroSnap™ Coliform can be used for both environmental surface samples and liquid or solid product samples, including filtered beverage samples and dairy products.

How long does it take to get results?

Enumeration results are typically available within 6 hours, while presence/absence results can be obtained in approximately 8 hours, enabling same-shift decisions.

Is MicroSnap™ Coliform an alternative to traditional plating methods?

Yes. MicroSnap™ Coliform provides rapid, quantitative results that correlate with traditional CFU counts, reducing the need for manual plating and colony counting in routine testing.

What equipment is required to run the test?

The test requires a Hygiena™ luminometer, such as EnSURE™ Touch, and access to SureTrend™ Cloud software for data storage, analysis, and reporting.

MicroSnap Coliform & E. coli with EnSURE Touch

MicroSnap Coliform & E. coli with EnSURE

MicroSnap Coliform for Milk Samples