Mesa Labs METSBBP16- Culture Media for Biological Indicators in Sterilization Testing

Tryptic Soy Broth with Bromocresol Purple (TSB-BP16)

Optimized for Rapid Spore Indicator Testing

TSB-BP16 is a specialized Tryptic Soy Broth containing Bromocresol Purple pH indicator, formulated specifically for use with Mesa’s Spore Strip biological indicators. It provides rapid, reliable detection of sterilization efficacy, making it ideal for laboratories validating steam sterilization processes.

Key Advantages

• Designed for compatibility with Mesa Spore Strips, including both single species (G. stearothermophilus) and dual species strips.
• Validated reduced incubation time of 24 hours at 60 °C ± 2 °C for steam sterilization cycles when used with Mesa’s Modified TSB-BP16.
• High-quality preparation in 16 mm x 100 mm Type I borosilicate glass tubes with polypropylene screwcaps and white rubber liners ensures safe handling and consistent results.

Model Information

• TSB-BP16 – 100 tubes per pack

Integrate additional testing tools such as the InSite™ Listeria – Environmental Listeria Species Test, which provides chromogenic liquid media and rapid presumptive screening for Listeria species in environmental samples. For sampling and surface hygiene verification prior to biological indicator incubation, sterile collection devices like the Hygiena Sample Sponge offer a convenient means to recover microbes and residues from large surface areas. In areas that are hard to access or where ergonomic sampling is critical, Hygiena Stick Sample Sponges combine a pre‑moistened sterile sponge with an extended handle to capture organisms from drains, equipment interfaces, and other challenging locations.

Q&A

What makes TSB-BP16 suitable for spore strip testing?

The broth includes Bromocresol Purple as a pH indicator, which changes color in response to bacterial growth. This allows for rapid visual confirmation of spore viability following sterilization cycles.

Can TSB-BP16 reduce incubation time?

Yes, when used with Mesa Spore Strips, the validated incubation time is 24 hours at 60 °C ± 2 °C, speeding up sterilization verification without compromising accuracy.

How is the media packaged for laboratory use?

Each tube is 16 mm x 100 mm, made of Type I borosilicate glass, sealed with a polypropylene screwcap and white rubber liner for secure handling and contamination prevention.

Are there similar biological indicator media available?

• TSB-BP8 – Tryptic Soy Broth with Bromocresol Purple, 8 mL tubes
• TSB Classic – Standard Tryptic Soy Broth for general microbial testing
• TSB-BP50 – Large volume 50 mL TSB with Bromocresol Purple for batch testing